Staphylococcus: Staphylococci are Gram positive cocci, 0.5-1.5 m in diameter, which occurs in irregular grape-like clusters. 3 None: Non motile, Non spore forming and typically Non encapsulated. Catalase positive. Facultative anaerobes. Grow on simple media (i.e. non fastidious). Manal Al Khulaif Gram-positive cocci are included among some of the most significant human bacterial pathogens: primary pathogens such as Staphylococcus aureus, Streptococcus pyogenes, and Strep. pneumoniae, along with species of lower virulence such as Staph. epidermidis, Staph. saprophyticus and Enterococcus faecalis
A report showing Gram positive cocci appearing in clusters has an indication that after a culture test, staphylococcus species of bacteria will be identified. This is an initial evaluation that shows the likelihood of a true pathogen staphylococcus aureus will be present or there is will be skin contaminant indicated by the coagulase negative staphylococci (CNS) MCQ on Gram Positive Cocci (Streptococcus pyogenes, Streptococcus pneumoniae, Staphylococcus aureus) 1) A 2 days old premature baby boy born to a 22-year-old woman developed a pneumonia, the mother was healthy but after the premature delivery she showed the symptoms of urinary tract infection. The blood specimen was taken from the baby, beta. These ubiquitous gram-positive cocci are commonly found on the skin and nasal mucosa, with 20% to 30% of the population being normal carriers of this bacterium. 81 S. aureus produces toxins and enzymes that form the basis of the lesions produced by this pathogen—a pyogenic exudate or an abscess. 81,114 Staphylococcal infections are usually.
•Staphylococci are gram-positive cocci •In Greek; staphyle - Bunch of grapes Kokkus - Berry •Spherical cells arranged in irregular clusters (grape like clusters) •Common inhabitant of the skin and mucous membranes •Lack spores and flagella •May have capsules •Catalase-positive •About 40 specie Staphylococcus epidermidis is a gram-positive, catalase-positive, coagulase-negative cocci in clusters and is novobiocin sensitive. S. epidermidiscommonly infects prosthetic devices and IV catheters producing biofilms. Staphylococcus saprophyticus is novobiocin resistant and is a normal flora of the genital tract and perineum There are two medically important genera of gram-positive cocci: Staphylococcus and Streptococcus. Two of the most important human pathogens, Staphylococcus aureus and Streptococcus pyogenes, are described in this chapter. Staphylococci and streptococci are nonmotile and do not form spores. + Gram-positive cocci are circular or oval in shape. The term cocci, which means sphere, indicates the bacteria are generally round. The following types are gram-positive cocci: Staphylococcus Gram Positive Cocci Anaerobes Streptococci Gram Negative Bacilli Staphylococci Gram Negative Coccobacilli Atypicals Bacteria Clostridium difficile Mycoplasma pneumoniae, Chlamydophila spp Legionella pneumophila Amoxicillin-clavulanate Cefoxiti
Gram-Positive Cocci Prof. Dr. Asem Shehabi Faculty of Medicine University of Jordan Gram-psitive cocci Micrococcaceae family.. - A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 43bd6d-MjAz Start studying Gram Positive Cocci: Staphylococcus. Learn vocabulary, terms, and more with flashcards, games, and other study tools Our Instagram pagehttps://www.instagram.com/invites/contact/?i=mi2yngr0vwrz&utm_content=m81zvo Staphylococcus is a genus of Gram-positive bacteria in the family Staphylococcaceae from the order Bacillales. Under the microscope, they appear spherical (cocci), and form in grape -like clusters. Staphylococcus species are facultative anaerobic organisms (capable of growth both aerobically and anaerobically) Staphylococci are Gram positive cocci of uniform size, occurring characteristically in groups but also singly and in pairs (see colour plate 24). They are non-motile. Blood agar, chocolate (heated blood) agar: S. aureus produces yellow to cream or occasionally white 1-2 mm in diameter colonies after overnight incubation
Gram-positive cocci Two pathogenic bacteria are G+ve B: Staphylococci and Streptococci. 1. Staphylococci: I- General features: 1. Staphylococci are G+ve cocci (spherical or grapes shape). 2. Are non motile, non capsulated, non spore forming. 3. Staphylococci are oxidase negative & catalase positive which one feature that distinguishes from. Gram Positive Cocci Identification Laboratory testing algorithm for Streptococcus spp , and Staphylococcus aureus, MRSA, S. epidermidis, S. saprophyticus , and S. lugdunensis Hover over the animation to start / stop rotatio
BACTERIOLOGY. Lecture. GRAM POSITIVE COCCI Staphylococcus Streptococcus. LEARNING OBJECTIVES At the end of this unit, the student is able to: 1. Describe Staphylococcus & Streptococcus in terms of their general properties and pathogenesis 2. Discuss the identification of characteristics in terms of morphology, culture media, colony characteristics, biochemical test, and serological test 3 Gram-positive cocci, such as Staphylococcus and Streptococcus, are contagious. Staphylococcus aureus, for example, is a common cause of hospital-acquired pneumonia, surgical wound infections, endocarditis, osteomyelitis and skin infections. The spread of bacterial infection can be airborne, by directly using infected intravenous catheters and.
Gram positive cocci in clusters Staphylococci Catalase. 14 Beta-hemolyt ic Alpha-hemolyt ic Non-hemolyt ic. 15 Gram O2 Arrangment Example Diseases Gram Positiv e Cocci Gram + cocci in chains Catalase negative Strep.pneumoniae Alpha hemolysis Pneumonia meningitis Group A strept Beta hemolysi Les staphylocoques sont des bactéries de type cocci à Gram positif, qui se retrouvent fréquemment chez les personnes en bonne santé, habituellement dans la muqueuse du nez
Staphylococcus is a genus of Gram-positive bacteria in the family Staphylococcaceae in the order Bacillales. Under the microscope, they appear spherical (cocci), and form in grape-like clusters. Staphylococcus species are facultative anaerobic organisms (capable of growth both aerobically and anaerobically) Gram positive cocci. Enterococcus Mechanisms of antibiotic resistance in enterococci Microbiology of enterococci Treatment of enterococcal infections Vancomycin-resistant enterococci: Epidemiology, prevention, and control Staphylococcus aureus bacteremia with reduced susceptibility to vancomyci The role of Gram-positive cocci as agents of foodborne illness is described along with methods for their control. The chapter focuses primarily on staphylococcal food poisoning caused by Staphylococcus aureus, but the enterococci and pathogenic streptococci that can be transmitted by food are also discussed Pathogenic Gram-Positive Cocci (Staphylococci) * * * * * * * * * * * Stain purple when gram-stained Can be categorized into 2 major groups Genera of cocci-shaped - A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3da3f5-YTU2 Gram positive cocci in clumps Belongs to family of Micrococcaceae non-motile, non-spore forming and glucose fermenter Most virulent species of Staphylococcus b-hemolytic on BAP Halophilic (can grow in presence of 7.5-10% NaCl) Produces a golden yellow pigment (staphyloxanthin) Toxin-Induced diseases: food poisoning; scalded skin syndrome (SSS) o
Staphylococcus and Streptococcus are both Gram positive organisms and cocci in shape. They are Non-motile, Non-Sporing and Facultative anaerobes. But they possess some of the differences which are as follows: S.N: Characteristics: Staphylococcus Gram-positive cocci: Staphylococcus aureus is a gram-positive, catalase-positive, coagulase-positive cocci in clusters. S. aureus can cause inflammatory diseases, including skin infections, pneumonia, endocarditis, septic arthritis, osteomyelitis, and abscesses There are a wide variety of gram-positive cocci in nature. Common to all is that they are seen as small, round, blue/purple stained bacteria under the microscope when stained with gram staining. In the medical field, 3 families of gram-positive cocci are of particular interest due to their ability to cause diseases in humans Staphylococcus aureus is the most pathogenic species in the group. The virulence of Staphylococcus aureus is due to a number of enzymes or toxins that some strains can produce.MICROCOCCUS: Gram-positive cocci arranged as single cells, pairs or irregular clusters The classification of Gram-positive cocci is based on bacterial staining as seen on the Gram stain. They form clusters (staphylococcus) or chains (streptococcus). Clustered Gram-positive cocci can be further differentiated based on the coagulase and catalase reactions (see Figure 1 below) which can be performed approximately 24 hours after.
gram positive cocci aerobic gram positive rods gram negative organisms anaerobes. Aerobic Gram-positive cocci Action on glucose Oxidative Micrococcus (pairs/clusters) Positive Staphylococcus (clusters) Coagulase Fermentative Catalase Negative Streptococcus Enterococcus (pairs and chains) Group A* Streptococcus pyogenes Beta-haemolytic Group. GRAM POSITIVE COCCI STAPHYLOCOCCI AND STREPTOCOCCI. LECTURE №8. Слайд 2. Текст слайда: RESPIRATORY INFECTIONS. Respiratory infections are diseases that transmitted mainly by airborne way and by dust. At the reception of a dentist, respiratory infections are one of the most frequently transmitted groups of infections, an. Gram-positive bacteria. Gram-positive cocci in clusters. Staphylococcus aureus and Staphylococcus epidermidis on mannitol salt agar Mannitol salt agar is a commonly used growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others
Staphylococci-Introduction Staphylococci are gram-positive cocci In Greek; staphyle - Bunch of grapes Kokkus - Berry Spherical cells arranged in irregular clusters (grape like clusters) Common inhabitant of the skin and mucous membranes Lack spores and flagella May have capsules Catalase-positive About 40 specie Staphylococcus genus positive or negative, mecA positive or negative No antibiotics - clinically monitor Clinical worsening, hypotension or second blood culture positive for gram-positive cocci in clusters Continues clinically stable and only 1 of 2 blood cultures positive No antibiotics indicated * See dosing guidance belo Bugs & Drugs Web Application. Bacterial Morphology Probable Organisms; Cocci in clusters/clumps; Staphylococcus sp
Staphylococcus aureus is the most dangerous of all of the many common staphylococcal bacteria. These gram-positive, sphere-shaped (coccal) bacteria (see figure How Bacteria Shape Up) often cause skin infections but can cause pneumonia, heart valve infections, and bone infections Staphylococcus aureus • Gram positive cocci • Arranged in grape like clusters • Cluster formation is d/t division in 3 planes and daughter cells remain in close proximity • May appear in singles, pairs or short chains • Some bacteria may have capsules 14
Pyogenic gram positive cocci Pyogenic gram positive cocci Staphylococcal infection - coagulase positive (S. aureus) - coagulase negative (S. epidermidis, S. saprophyticus ) Streptococcal infection - S. pyogenes (group A) - S. agalactiae (group B) - S. pneumoniae-S m. utans Staphylococcus spp. Streptococcus spp Gram-positive cocci are often isolated from clinical samples. It is important to be able to distinguish these organisms from one another because while some are normal flora, others can be pathogenic. Below are some commonly encounter gram-positive cocci. STAPHYLOCOCCUS: Gram-positive cocci, arranged in grape-like clusters. Catalase positive
Staphylococcus 5. Other gram-positive cocci that have developed antibiotic resistance include vancomycin-intermediate and vancomycin-resistant Staphylococcus aureus, Enterococcus faecium and community-acquired methicillin-resistant Staphylococcus aureus, according to Drugs.com. (reference.com); Some gram-positvie bacteria, such as Staphylococcus aureus and Streptococcus pyogenes, release. This chapter discusses mainly those Gram-positive cocci that were members of the former family Micrococcaceae prior to its redefinition. The genera have now been rearranged in different families: the Staphylococcaceae, the redefined Micrococcaceae, and the newly established Dermacoccaceae.The two last families comprise micrococci, clinically relevant species of which now belong to the genera. Anaerobic Gram positive cocci; Anaerobic Gram positive bacilli; Anaerobic Gram Negative Bacilli. Porphyromonas spp; Fusobacterium spp; Prevotella spp; Bacteroides spp; Spirochetes. Treponema spp Aerobic Gram negative bacilli and Staphylococcus aureus rarely cause dental infections. They may be the causative agents in patients with serious. Gram stain. the following information is not yet verified Gram positive cocci, 0.5-1.2 µm that occur in irregular grape-like clusters and, less often, single and in pairs, tetrads, and in short chains. Culture characteristics. the following information is not yet verified. Facultative anaerobi This plate demonstrated cloudy growth and yellow agar where the growth was present. Since MSA is a selective media, which selects for Gram-positive bacteria, a Gram stain was accomplished on this growth to determine if it was the Gram-positive bacteria. The Gram stain demonstrated Gram-positive cocci
Gram positive cocci (clusters) Normal skin flora * Catalase positive Coagulase negative * Non-hemolytic on blood agar * MSA - does not ferment mannitol Two imp. species: S. epidermidis S. saprophyticu Staphylococcus aureus (S. aureus) is a gram positive bacterium that when looked at under a microscope it appears to be a cluster of what looks like purple circles. This shape is known as cocci. When grown on a TSA plate, Staphylococcus aureus appears to be yellow to opaque in color Streptococcus pneumonia are gram positive diplococci or chain of cocci The colonies of Streptococcus pneumonia on blood agar and chocolate agar looks small, greyish, and mucoid and especially α-hemolysis (greenish surround the colonies
Gram Positive Cocci. List 3 ways to differentiate Staphylococcus spp. from Streptpcoccus spp. and the expected results for each. Gram Stain: Staph-cocci in clusters, Strep-cocci in pairs and chains. Nice work Can Gram-positive cocci be treated? Is Gram-positive cocci contagious? However, it is known that given the right circumstances, even if the staph infection arises from organisms present on one's body, if another person has direct contact with the infectious staph bacteria and has a break in the skin or mucous membranes, the organisms and potentially the disease can be contagious Study Pathogenic Micro: Gram Positive Cocci (Staph and Strep)- Packet 4 flashcards. Create flashcards for FREE and quiz yourself with an interactive flipper A total of 182 gram positive cocci obtained from consecutive clinical specimens were included, consisting of 19.7% methicillin resistant Staphylococcus aureus (MRSA), 41.7% methicillin sensitive S. aureus (MSSA), 8.7% methicillin-resistant coagulase-negative staphylococci (MRCNS), 17.5% methicillin-sensitive coagulase-negative staphylococci.
gram-positive cocci in chains. Culture of the pus grows small. β-hemolytic colonies that are catalase-negative and are inhibited. by bacitracin. These results indicate that the organism causing. her lesion is most likely: (A) Enterococcus faecalis. (B) Staphylococcus aureus. (C) Streptococcus agalactiae Gram-stain: Gram-positive : Microscopic appearance: Cocci in grape-like clusters, diplococci, cocci : Clinical significance: Staphylococcus epidermidis is part of human skin flora (commensal). It can also be found in the mucous membranes and in animals Gram positive cocci Genus: Staphylococcus spp The genus Staphylococcus contains about 30 species. Only some of them are important as human pathogens : 1-Staphylococcus aureus (Staphylococcus pyogenes) Is responsible for most Staphylococcal infections and aureus causes a variety of suppurative (pus-forming) infections. Nasal carriage occurs i
Staphylococcus Gram Stain This mixture consists of the gram-positive Staphylococcus albus (purple-stained spheres arranged in grape-like clusters), and the gram-negative Escherichia coli (red-stained rods scattered singly throughout the field). Shape: gram positive cocci, Bunch (cluster), Non spore forming, non motile Prof. Dr. AbD El-GAwAD M. Gram positive Cocci/ Streptococcus Streptococci are gram positive, nonmotile, and catalase negative. Clinically important genera include Streptococcus and Enterococcus (Figure 1A). They are ovoid to spherical in shape and occur as pairs or chains (Figure 2). Most are aerotolerant anaerobes because they gro
1. Gram-positive cocci in grape-like clusters. 2. Catalase positive; growth in 7.5% salt. 3. Ubiquitous distribution; part of normal flora; responsible for many nosocomial infections. B Coagulase-positive staphylococci (S. aureus) S. aureus: major cause of nosocomial and wound infections. S. aureus ferment mannose, other staph don't Common Characteristics of Staphylococcus Gram-positive cocci Catalase-positive Single, pairs, tetrads Short chains, grapelike clusters Nonmotile No spores Facultative anaerobes (most) Normal flora of skin and mucous membranes Nasal carriers of SA = up to 40% of population Pathogen Any body site Most often hospital-acquired (nosocomial), but can. 12 Fecalysis - Notes extracted from Ma'am Camille Mendoza, RMT 2. Phlebotomy - Notes taken from the lecture of Sir Antonio Pascua, Jr., RMT 3 Antimicrobial- Susceptibility-TEST 6 GRAM- Positive- Cocci- Family-OF- Streptococcaceae 7 GRAM- Negative- Cocci TOUR 19 - Week 17 - Lecture notes 1 Gram positive cocci are a group of microorganisms responsible for a variety of infections, including staphylococcal, or staph infections; streptococcal infections, such as strep throat; and pneumococcal infections, such as bacterial pneumonia. Gram positive cocci tend to cause infections only when they invade otherwise sterile or healthy areas
Gram Positive Cocci (GPC) Gram-positive bacteria in Gram stain are showing Gram-positive cocci singly, in pairs, chains, and in groups/clusters that indicate the possibility of an organism is Staphylococcus aureus as shown above image.Gram stain is a differential stain and therefore it uses to differentiate Gram-positive and Gram-negative bacteria Gram Positive Cocci. Micrococaceae. Micrococci. Staphylococci. Stomatococci. of grapes-butyrous-Mannital salt agar (yellow)-catalase positive-Characteristics that differentiate coagulase positive staphylococci . organism: Slide : tube : pyr : thermostable nuclease : VP (gram - ) MRS- resistant to Beta lactams and cephalosporin, imipenin. A - Staphylococcus Les staphylocoques sont des cocci à Gram positif qui tendent à se grouper en amas. Une espèce, Staphylococcus aureus (staphylocoque doré), tient une place très importante dans les infections communautaires et nosocomiales. I - Staphylococcus aureu